Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetFluorogenic caspase-3 substrate.

General description

Fluorogenic CPP-32/apopain substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Excitation max: ~400 nm; emission max: ~505 nm.

Fluorogenic caspase-3 substrate. Also acts as a substrate for granzyme B-processed ICE-LAP3. Reaction can be monitored by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore. Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Keramaris, E., et al. 2000. Mol. Cell Neurosci. 15, 368.Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.Kluck, R.M., et al. 1997. Science275, 1132.Talanian, J., et al. 1997. J. Biol. Chem.272, 9677.Chinnaiyan, A.M., et al. 1996. Curr. Biol.6, 897.Nicholson, D.W., et al. 1995. Nature376, 37.

Packaging

1, 5 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for 3 months at -20°C.

Sequence

Z-Asp-Glu-Val-Asp-AFC

Warning

Toxicity: Standard Handling (A)